Humans may have been enjoying cacao, the substance used to make chocolate, for much longer than experts had thought.

Researchers at the University of British Columbia in Canada have found that humans grew cacao trees and consumed cacao starting around 5,300 years ago.

They published their findings in the journal Nature Ecology & Evolution in late October.

The researchers found evidence of cacao’s use at an ancient village in the highlands of southeastern Ecuador. They examined the remains of very old objects at the Santa Ana-La Florida archeological site. The village was part of the Mayo-Chinchipe culture of the Andes.

Time has had little effect on the village and ceremonial center. The researchers were able to find a lot of evidence of the use of cacao.

Scientists had already mostly agreed that cacao was first domesticated in South America instead of Central America, as they once believed. But the new discovery shows cacao was domesticated about 1,500 years earlier than was known before.

The scientists found evidence of cacao being used at the site from 5,300 to 2,100 years ago.

Cameron McNeil is a professor at Lehman College in New York. She is editor of the book “Chocolate in Mesoamerica.” She told the Associated Press that Santa Ana-La Florida is “the earliest site now with domesticated cacao.” McNeil was not involved in the new research.

The University of British Columbia researchers found extremely small pieces from the cacao tree in the remains of containers and other objects, as well as genetic material from the tree.

They also identified a substance found in the cacao tree but not in its wild relatives. This suggests that humans grew the tree for food purposes.

A tropical tree known as Theobroma cacao produces large, round pods that contain the bean-like cacao seeds. A soft, light-colored substance covers each seed. Today, the seeds are cooked and turned into many chocolate products.

But thousands of years ago, cacao was used to make drinks.

Michael Blake is a professor of archaeology at the University of British Columbia who helped lead the study. He said that the objects on which cacao was found suggest a lot about how people used the substance at the time.

“They clearly drank it,” Blake told the Reuters news service.

There is no established history of indigenous populations in South or Central America using cacao to make chocolate the way people do now, the researchers reported. Indigenous populations in the upper Amazon area today still use cacao to make special drinks, they added.

Archeological evidence suggests cacao domestication moved into Central America and Mexico about 4,000 years ago. It is not clear how cacao’s use spread between South and Central America.

But by the time Spanish explorers arrived in Central America in the late 1400s, people were using it to make hot and cold chocolate drinks with spices.

By the 1580s, Spain began importing cacao and spreading it to other European countries. By the 1800s, technology developed in the Netherlands made it possible to turn cacao into a solid chocolate product.

Michael Laiskonis teaches chocolate-making classes at the Institute of Culinary Education in New York. He said he has observed a growing interest in natural cacao tastes.

He said he introduces his students to the history of chocolate. He tells his students, for example, about a recipe from the 1600s that mixed Mayan and Aztec versions of cacao drinks with European influences.

Laiskonis said chocolate is “something that’s always been transforming.”